FIG 2.
Effect of gp120 layer mutations on neutralization by CD4 and CD4mc. Recombinant HIV-1 strains expressing luciferase and bearing wild-type or mutant CRF01_AE Envs (92TH023 and CM244 isolates) were normalized by reverse transcriptase activity. Normalized amounts of viruses were incubated with serial dilutions of sCD4 (A and B), BNM-III-170 (C and D), or M48U1 (E and F) at 37°C for 1 h prior to infection of Cf2Th-CD4/CCR5 cells. Infectivity at each dilution of sCD4 or CD4mc tested is shown as the percentage of infection without sCD4 or CD4mc for each particular mutant. Quadruplicate samples were analyzed in each experiment. Data shown are the means of results obtained in at least 3 independent experiments. The error bars represent the standard deviations. Neutralization half-maximal inhibitory concentration (IC50) values are summarized in panel G.