FIG 7.

Residue 375 modulates Env sensitivity to different families of CD4mc. (A to C) Chemical structures of representative members of the different families of CD4mc tested. (D to I) Cell surface staining of primary CD4⁺ T cells infected with CH58 or JRFL viruses and their variants with anti-Env 17b MAb (D to F) or HIV⁺ sera (G to I) was performed. Shown in panels D and G are histograms depicting representative 17b and HIV⁺ serum staining results. The graphs shown represent the fold increase in mean fluorescence intensities (MFI) in the presence of CD4mc calculated on the infected (p24⁺) population obtained with 17b (E and F) or with 5 different HIV⁺ sera (H and I). These results were obtained in at least 3 independent experiments. Error bars indicate means ± SEM. Statistical significance was tested using a paired t test or a Wilcoxon signed-rank test based on statistical normality (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, nonsignificant).