TABLE 1.
Evolved mutant clones genotyped by WGS demonstrate increased TOB resistance (MIC) relative to the ancestral clonea
| Species | Genotype | MIC (mg/liter) |
Fold changeb |
|
|---|---|---|---|---|
| Median | Range | |||
| A. baumannii | Ancestor | 1.0 | 1.0–2.0 | |
| fusA1 R59H | 4.0 | 2.0–4.0 | 4.0 | |
| fusA1 G460V | 4.0 | 4.0–8.0 | 4.0 | |
|
cyoA V53V ptsP indel of nt 1742/2295 ACX60_RS12470 A173D |
4.0 | 2.0–4.0 | 4.0 | |
| fusA1 G460V ptsP indel of nt 1340–1368/2295 | 8.0 | 8.0–8.0 | 8.0 | |
| fusA1 G460V ptsP indel of nt 1788/2295 | 8.0 | 8.0–8.0 | 8.0 | |
|
fusA1 F535L ptsP Δ2bp 587–588/2295 ACX60_RS04175 R62C |
8.0 | 8.0–16.0 | 8.0 | |
| P. aeruginosa | Ancestor | 0.5 | 0.25–1.0 | |
| fusA1 Q129H | 1.5 | 1.0–2.0 | 3.0 | |
| fusA1 N592I | 2.0 | 2.0–2.0 | 4.0 | |
| fusA1 F596L | 2.0 | 1.0–2.0 | 4.0 | |
| fusA1 Q678L | 2.0 | 1.0–2.0 | 4.0 | |
| fusA1 R680C | 1.0 | 1.0–2.0 | 2.0 | |
| ptsP Δ42bp (deletion of nt 1846–1887/2280) | 1.0 | 1.0–2.0 | 2.0 | |
| ptsP Δ14bp (deletion of nt 1296–1309/2280) | 1.5 | 1.0–2.0 | 3.0 | |
| fusA1 Q678L ptsP R301C | 4.0 | 1.0–4.0 | 8.0 | |
| fusA1 T456A ptsP V661E | 2.0 | 1.0–4.0 | 4.0 | |
| fusA1 Q563L ptsP E335* | 4.0 | 2.0–4.0 | 8.0 | |
| fusA1 R680C ptsP E335* | 4.0 | 2.0–4.0 | 8.0 | |
| fusA1 T671A ptsP Δ1bp (deletion of nt 1122/2280) | 4.0 | 2.0–8.0 | 8.0 | |
| fusA1 N592I orfN Δ1bp (deletion of nt 148/1017) | 2.0 | 2.0–4.0 | 4.0 | |
a
Data are from 4 replicates.
b
Fold change in the MIC from that for the ancestor.