Fig. 4.

Increases in S-glutathionylation in lung tissues from patients with IPF. Lung sections were deparrafinized, rehydrated, permeabilized and reduced protein thiols blocked with N-ethyl maleamide (NEM). Sections were then subjected to GLRX-catalyzed protein cysteine labeling in order to detect regions of PSSG, as described in the text. Red = PSSG, Blue = DAPI counterstain Note the increases in PSSG in lungs from IPF patients (n = 4), compared to non-IPF controls (n = 4). This image was first published in Nature Medicine. 2018 Aug; 24 (8):1128–1135. https://doi.org/10.1038/s41591-018-0090-y. Epub 2018 Jul 9. by Anathy V et al. and was reproduced with permission from Nature Medicine [68]. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)