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. 2020 May 25;15:1–9. doi: 10.1016/j.reth.2020.03.011

Fig. 2.

Fig. 2

DAZAP2 modified the cell proliferation rate in human iPSCs derived from Edom cells. (A) Immunofluorescence staining of DAZAP2 in undifferentiated induced pluripotent stem cells (iPSCs) derived from Edom22 cells. Green signals indicate DAZP2 protein. DAPI staining is shown in blue. Scale bar: 100 μm. (B) Colony of DAZAP2 overexpressing Edom–iPSCs with normal morphologies. Red signals indicated DAZAP2-overexpressing cells. Scale bar: 100 μm. (C) Undifferentiated markers, including OCT3/4, NANOG, and stage-specific embryonic antigen 4 (SSEA4), were normally observed in DAZAP2-overexpressing Edom–iPSCs. DAPI staining is shown in blue. Scale bar: 100 μm. (D) Undifferentiated marker gene expression, including OCT3/4, NANOG, SOX2, TRIM28, TDGF1, DNMT3B and STAT3, were comparable between control and DAZAP2-overexpressing (OE) Edom–iPSCs. Precursor of hsa-miR-302 cluster were also comparable between control and DAZAP2-overexpressing (OE) Edom–iPSCs. (E) The cell proliferation rate was significantly decreased in DAZAP2-overexpressing Edom–iPSCs compared to control Edom–iPSCs. ∗∗P < 0.01.