Table 1.

Genomic Integration Frequencies of Alt-R EE

Gene	gRNA Formulation	Alt-R EE Insertion (%)	SD
RAG2	Alt-R sgRNA	0.009	0.01
	Alt-R 2-part XT RNA	0.013	0.01
	Alt-R 2-part RNA	0.035	0.03
RAG1	Alt-R sgRNA	0.016	0.02
	Alt-R 2-part XT RNA	0.052	0.05
	Alt-R 2-part RNA	0.017	0.02

The gDNA of CD34⁺ HSPCs was examined by NGS for the integration of the Alt-R EE, following genome editing with 4 μM RNP complexes, as described in Figure 1. SD, standard deviation.