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. 2020 May 26;40(5):BSR20200723. doi: 10.1042/BSR20200723

Figure 4. SNHG16 was a sponge of miR-542-3p to isolate HNF4α.

Figure 4

(A) The binding sites between miR-542-4p and SNHG16 were predicted by starBase v2.0. (B,C) Dual-luciferase reporter assay was carried out to assay the interaction between miR-542-3p and SNHG16. (D,E) The level of miR-542-3p in NB tissues and cell lines was identified. (F) qRT-PCR was performed to verify the correlation between miR-542-3p level and SNHG16 level. (G) The role of si-SNHG16 and pcDNA-SNHG16 in regulating miR-542-3p level was verified. (H) Prediction of the complementary sequences between miR-542-3p and HNF4α were shown. (I,J) The relationship between miR-542-3p and HNF4α was clarified using dual-luciferase reporter assay. (K) The correlation between miR-542-3p level and HNF4α level was analyzed. (L,M) The expression of HNF4α was detected adopting Western blot. *P<0.05.