Fig. 2.

Cytosol/mitochondria partitioning of sinapine and sinapic acid in cardiomyocytes. Myocytes were incubated for 1 h (left panel) or 2 h (right panel) with different concentrations of sinapine (A, B) or sinapic acid (C, D) (2, 20, and 60 μM). After fractional separation, the amounts of sinapine (grey column) or sinapic acid (black column) were measured by HPLC in the cytosol (A, C) or in isolated mitochondria (B, D). Conditions without drug incubation served as a negative control. (n = 6 experiments with sinapine, n = 4 experiments with sinapic acid).