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. 2020 May 13;13:100226. doi: 10.1016/j.ynstr.2020.100226

Fig. 3.

Fig. 3

Anatomical description of brain regions regulated in the Vehicle-SPS and drug-pretreated groups compared to the Vehicle-Sham group. The data are reported as bar graph representations (mean + S.E.M.) or anatomical visualizations in which neuronal activation (increase in c-fos+ cell counts) or inhibition (decrease in c-fos+ cell counts) are visualized, respectively, as red or green signals overlaid on a grayscale image of a mouse brain. (A) SPS induced activation in the BST, PVH and MPN as well as in the deep layers of the somatosensory cortex (panel SPS). Pretreatment with prazosin only partially reduced the stress-induced activation in these structures (panel SPS + Prazosin), while the activation was nearly entirely abolished by pretreatment with ACH-000029 (panel SPS + ACH). (B) Prominent activation evoked by the SPS protocol is seen in the CEA, MEA, LA, PH, PVT, LH, and the deep layers of the AUD (panel SPS). Pretreatment with prazosin partially reduced the activation in the LH, CEA and PVT, while the activation was almost fully abolished by pretreatment with ACH-000029. Inhibition in the PIR, BLA and BMA was more pronounced after prazosin pretreatment. (C) SPS induced activation in the CEA, MEA, and HY (hypothalamus), the PVT (paraventricular nucleus of the thalamus) and the IMD (intermediodorsal nucleus of the thalamus) as well as in the SS. Prazosin partially reduced and ACH-000029 entirely abolished the stress-induced activation in these structures. (D) SPS-induced activation is seen in the PAG and deep layers of the AUD (panel SPS). Prazosin pretreatment partially reduced the stress-induced activation in PAG and AUD, while ACH-000029 completely abrogated this activation. Inhibition in the HPF was seen in all comparisons, while inhibition in the PIR, ENTl, and COA was more intense in mice pretreated with prazosin than in mice pretreated with ACH-000029. (E) Brainstem activation evoked by the SPS protocol is seen in the PAG and the CUN (cuneiform nucleus). CUN activation was partially reduced by prazosin but nearly entirely abolished by ACH-000029 pretreatment. ENTm inhibition was prominent in mice treated with prazosin. *q < 0.05, **q < 0.01, ***q < 0.001. Statistical comparisons were made against the Sham-Vehicle group. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)