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. Author manuscript; available in PMC: 2020 Sep 16.
Published in final edited form as: Chem Res Toxicol. 2019 Aug 28;32(9):1801–1810. doi: 10.1021/acs.chemrestox.9b00146

Figure 6:

Figure 6:

Recovery of activity in BChEG117H and loop mutants following exposure to 10 mM OP inhibitor (paraoxon, DFP or EthP). Enzyme was allowed to react with the OP toxicant for one minute and then diluted 400-fold into a solution containing substrate (BTChI, 1 mM). Figure 6A shows a representative plot of raw enzyme activity (ΔA412) following dilution including the reactivation and recovery phases. Figure 6B shows the mean ± SEM of k3 values. In the case of DFP, 100% reactivation was not achieved, thus reported k3 values have been corrected based on Hovanec et al43.