. Author manuscript; available in PMC: 2020 Sep 16.

Published in final edited form as: Chem Res Toxicol. 2019 Aug 28;32(9):1801–1810. doi: 10.1021/acs.chemrestox.9b00146

Table 2: Comparison of the substrate kinetics parameters of BChE_G117H and loop mutants using paraoxon as the substrate.

Kinetic analysis was determined using non-linear regression with the classic Michaelis-Menten equation. Values are reported as mean ± SEM. An asterisk indicates a significant difference compared to BChE_G117H.

	k_cat ^a (min⁻¹)	K_M (μM)	k_cat/K_M (min⁻¹ M⁻¹)
G117H	0.244 ± 0.03	107.6 ± 28.1	2.2 × 10³
ENI	0.071 ± 0.003*	75.82 ± 9.3	0.9 × 10³
ENA	0.078 ± 0.004*	19.72 ± 5.3*	3.9 ×10³
ENG	0.089 ± 0.005*	24.23 ± 6.0*	3.6 ×10³
ENR	0.092 ± 0.005*	77.62 ± 12.2	1.2 × 10³
ENT	0.076 ± 0.005*	23.59 ± 6.1*	3.2 × 10³

k_cat = V_max/[enzyme active site]

Table 2: Comparison of the substrate kinetics parameters of BChEG117H and loop mutants using paraoxon as the substrate.

Table 2: Comparison of the substrate kinetics parameters of BChE_G117H and loop mutants using paraoxon as the substrate.