Subject	Proteomics
Specific subject area	Comprehensive proteome profiling of “normal” human cerebrospinal fluid (CSF) using mass spectrometry (MS).
Type of data	Liquid chromatograply tandem mass spectrometry (LC–MS/MS) data.
How data were acquired	LC-MS/MS acquisition on a nanoElute LC system coupled to a timsTOF Pro mass spectrometer.
Data format	Raw and processed.
Parameters for data collection	We re-analyzed samples previously analyzed in a report by Macron et al.[1]. A commercial pool of “normal” human CSF samples was prepared according to a previously published proteomic workflow [2,3], described in the following Method section. Sample fractionation was used.
Description of data collection	LC-MS/MS analyses of the resulting 24 fractions were performed using a nanoElute LC system, coupled to a timsTOF Pro mass spectrometer, to evaluate the instrumental performances for the proteomic profiling of CSF with respect to other LC-MS technologies [1]. Mass spectral data were searched using Mascot and X! Tandem search engines before being visualized and validated with the Scaffold software.
Data source location	Nestlé Research, 1015 Lausanne, Switzerland.
Data accessibility	Protein and peptide lists are provided in Supplementary Table S1. Repository name: ProteomeXchange Consortium. Data identification number: PXD018369.