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. 2020 May 27;11:208. doi: 10.1186/s13287-020-01729-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — CD133 is highly expressed in human embryonic stem cells. a Analysis of CD133 mRNA expression levels of two human embryonic stem cell (WA26 and RuES2, passage 20), HCT116, U2OS, HeLa, and HEF by RT-qPCR. Two paired primers were used. Bars indicate mean ± SD (n ≥ 2). b The protein level of CD133 in human cell lines determined by Western blot (immunoblotting) using a commercial CD133 antibody (Biorbyt, orb10288). c Flow cytometry analysis of these human cells using a commercial anti-CD133/1 (AC133)-APC conjugated antibody (Miltenyi Biotec, 130-098-129). The bar plot shows the percentage of CD133-positive cell in each cell line