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. 2020 Apr 22;44(1):156–164. doi: 10.3892/or.2020.7592

Figure 1.

Figure 1.

The forced expression of Cav2.1 mt-Q26 suppresses the proliferation of the SH-SY5Y cells compared with the Cav2.1 wt-Q13 and the EV transfectants. (A) Diagram of the structures of Cav2.1 wt and Cav2.1 mt molecules. (B) Sanger sequencing result of the Cav2.1 wt and Cav2.1 mt-expressing plasmid. The sequences in the red boxes indicate that the 13 and 26 CAG repeats were successfully synthesized and inserted into the pcDNA3.1 vector. (C) mRNA expression levels of Cav2.1 wt and Cav2.1 mt in the transfected cells were confirmed by qPCR (t-test). (D) WST-1 assay indicated that the forced expression of Cav2.1 mt-Q26 suppressed SH-SY5Y cell proliferation (LSD test). **P<0.01. Cav2.1 mt-Q26, mutant-type Cav2.1 with 26 polyQ repeats; Cav2.1 wt-Q13, wild-type Cav2.1 with 26 polyQ repeats; cAG, cytosine-adenine-guanine; EV, empty vector; NS, no significance.