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. 2020 May 27;11:203. doi: 10.1186/s13287-020-01702-x

Fig. 3.

Fig. 3

Retinal ganglion cell (RGC) survival at days 21 and 60 post-treatment. a Retinal ganglion cell (RGC) survival visualized by immunostaining for Brn3a. b Quantification of Brn3a+ cells by immunostaining in (a). Brn3+ cells were counted in 12 areas and averaged to estimate the RGC survival per mm2 in four to six optic nerves per group. Data are given as mean ± SD and analyzed by one-way ANOVA and post-hoc Tukey’s test. ***P < 0.001. c Immunostaining of retina for Tuj1 that shows the Brn3a expression trend