Fig. 6.

Cistauosis in retinal ganglion cells (RGCs) post-treatment. a On day 3, we observed obvious signs of cis p-tau formation in the retinal ganglion cell (RGC) layer of the injured retina. At 21 days post-injury, double-stained cis p-tau/Tuj 1 showed numerous axons that contained cis p-tau, while it was also observed in the retinal vascular system. b Western blot of cis p-tau, Tuj 1, pin 1, and β-actin. c Optical densitometry of cis p-tau, Tuj1, pin1 which has normalized to β-actin. An increase in cis p-tau resulted in decreased Tuj1 levels and vice versa. Data are given as mean ± SD for four samples per group. The data were analyzed by the un-paired t test. *P < 0.05, **P < 0.01. d Single laser confocal plane shows the degenerating double-positive cis p-tau and Tuj1 neurons observed in the vehicle group 60 days post-treatment. The axons in the extracellular vesicle (EV) group are absent in cis p-tau