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. 2020 Apr 21;44(1):213–223. doi: 10.3892/or.2020.7590

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Establishment of stably transfected strains of HCT116, DLD-1, SW480 cell lines. (A) Western blot analysis of RAD18 expression in the three stably transfected strains. (B) Results of the quantitative analysis of the western blotting by ImageJ. (C) Results of RT-PCR of RAD18 in the three stably transfected strains. ***P<0.001, Student's t-test. RAD18sh, RAD18-knockdown cells; SW480 RAD18, RAD18-overexpressing SW480 cells. RAD18, E3 ubiquitin protein ligase.