Table 1.
Descriptive statistics of DNA quantity and quality for original DNA and post-WGA samples amplified by REPLI-g®, MALBACTM, PicoPLEX®, and GenomePlex® kits
| Descriptive statistics, Mean (CV) | |||||
|---|---|---|---|---|---|
| Original DNA | REPLI-g® | MALBACTM | PicoPLEX® | GenomePlex® | |
| DNA concentration (ng/µl) | 1.05 (± 0.73) | 415.00 (± 0.22) | 15.30 (± 0.20) | 32.35 (± 0.14) | 2.85 (± 1.14) |
| Fold-change of DNA concentration | 745.00 (± 1.09) | 30.51 (± 1.14) | 60.31 (± 1.11) | 3.64 (± 1.04) | |
| Estimated parasites DNA quantity (genome/µl) | 7789.16 (± 1.90) | 66261.49 (± 2.41) | 374310.95 (± 1.80) | 2095.4563 (± 1.18) | 1052.71 (± 1.08) |
| Fold-change of parasites DNA quantity | 745.00 (± 1.09) | 30.51 (± 1.14) | 60.31 (± 1.11) | 3.64 (± 1.04) | |
| Nanodrop (260/280) | 0.97 (± 0.29) | 1.86 (± 0.01) | 1.88 (± 0.02) | 1.80 (± 0.01) | 1.44 (± 0.11) |
| Ratio (P. falciparum(genome/µl)/Total DNA(ng/µl)) | 8123.64 (± 1.40) | 124.11 (± 2.33) | 21,924.46 (± 1.85) | 63.37 (± 1.21) | 732.43 (± 1.38) |
The quality of pre-WGA and post-WGA samples were determined using Nanodrop™. The ratio of absorbance at 260/280 was used to assess DNA purity. Data are represented as Mean (± CV)