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. 2020 Apr 23;44(1):77–90. doi: 10.3892/or.2020.7593

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — DJ-1 is highly expressed in CRC tissues and cell lines. (A) DJ-1 mRNA in FHC and 7 CRC cell lines was analyzed using RT-qPCR. Data indicated that DJ-1 mRNA in FHC was lower than that in the CRC cell lines (**P<0.01). (B and C) DJ-1 protein was detected by western blot analysis. Actin served as an internal control of the protein level. The expression of DJ-1 was higher in the 7 CRC cells than that noted in the FHC cells. Densitometric analysis is presented as mean ± SD of 3 separate experiments (*P<0.05, **P<0.01). (D and E) Expression of DJ-1 was increased in cancer tissues (C1-C8) when compared with that noted in the paired normal colonic tissues (N1-N8) by western blot analysis. Densitometric analysis is presented as mean ± SD of 3 separate experiments (*P<0.05, **P<0.01). CRC, colorectal cancer.