Figure 5.

PGK1 is a miR-638 target. (A) Forecasted binding sequences between miR-638 and PGK1 3′UTR. (B) Examination of luciferase activity at 48 h following the co-transfection of HAECs with PGK1-WT or PGK1-MUT reporter and miR-638 and miR-con. (C) Assessment of the effects of miR-638 mimics and miR-638+NEAT1 on PGK1 protein expression using western blot assay at 48 h after HAEC transfection. (D) PGK1 expression in the serum of 24 healthy controls and 24 AS cases. (E and F) Assessment of the relationship between PGK1 and miR-638 or NEAT1 in the serum of 24 AS cases. *P<0.05, **P<0.01 and ***P<0.001. PGK1, phosphoglycerate kinase 1; HAECs, human aortic endothelial cells; NEAT1, nuclear paraspeckle assembly transcript 1; AS, atherosclerosis.