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. 2020 May 7;44(1):115–125. doi: 10.3892/or.2020.7605

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — miR-638 adjusts HAEC proliferation and apoptosis through Akt/mTOR signaling. (A) HAECs were treated with the control, miR-638 inhibitor, miR-638 mimics, si-NEAT1, and si-NEAT1+miR-638 inhibitor mimics+PGK1 after 1 day of ox-LDL (50 µg/ml) stimulation, and mTOR, p-mTOR, AKT, and p-AKT expression levels were assessed. (B) Cell proliferation abilities were determined. (C) Colony formation ability. (D) Expression levels of PCNA and Ki-67. miR-638 adjusts HAEC proliferation and apoptosis through Akt/mTOR signaling. (A) HAECs were treated with the control, miR-638 inhibitor, miR-638 mimics, si-NEAT1, and si-NEAT1+miR-638 inhibitor mimics+PGK1 after 1 day of ox-LDL (50 µg/ml) stimulation, and mTOR, p-mTOR, AKT, and p-AKT expression levels were assessed. (B) Cell proliferation abilities were determined. (C) Colony formation ability. (D) Expression levels of PCNA and Ki-67. (E) Cells undergoing apoptosis. (F) Caspase-3 activity. (G) Bcl-2 and Bax expression levels. *P<0.05, **P<0.01 vs. control group, ^#P<0.05 vs. miR-638 mimics group. HAEC, human aortic endothelial cell; NEAT1, nuclear paraspeckle assembly transcript 1; ox-LDL, oxidized low-density lipoprotein; PCNA, proliferating cell nuclear antigen.

Figure 6. — miR-638 adjusts HAEC proliferation and apoptosis through Akt/mTOR signaling. (A) HAECs were treated with the control, miR-638 inhibitor, miR-638 mimics, si-NEAT1, and si-NEAT1+miR-638 inhibitor mimics+PGK1 after 1 day of ox-LDL (50 µg/ml) stimulation, and mTOR, p-mTOR, AKT, and p-AKT expression levels were assessed. (B) Cell proliferation abilities were determined. (C) Colony formation ability. (D) Expression levels of PCNA and Ki-67. miR-638 adjusts HAEC proliferation and apoptosis through Akt/mTOR signaling. (A) HAECs were treated with the control, miR-638 inhibitor, miR-638 mimics, si-NEAT1, and si-NEAT1+miR-638 inhibitor mimics+PGK1 after 1 day of ox-LDL (50 µg/ml) stimulation, and mTOR, p-mTOR, AKT, and p-AKT expression levels were assessed. (B) Cell proliferation abilities were determined. (C) Colony formation ability. (D) Expression levels of PCNA and Ki-67. (E) Cells undergoing apoptosis. (F) Caspase-3 activity. (G) Bcl-2 and Bax expression levels. *P<0.05, **P<0.01 vs. control group, ^#P<0.05 vs. miR-638 mimics group. HAEC, human aortic endothelial cell; NEAT1, nuclear paraspeckle assembly transcript 1; ox-LDL, oxidized low-density lipoprotein; PCNA, proliferating cell nuclear antigen.