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. 2020 Apr 28;44(1):126–138. doi: 10.3892/or.2020.7596

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Copyright: © Thrasyvoulou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — VL30 retrotransposition induces a mesenchymal phenotype associated with the protein expression of EMT markers in HC11 cells. (A) Fields of control HC11 or J3B1A cells and respective retrotransposition-positive clone cells (magnification, ×20) grown in normal culture dishes. HC11/cl.19 and J3B1A/cl.8 panels represent cells with an induced and non-induced mesenchymal phenotype, respectively. (B) Western blot analysis of whole protein lysates from NIH3T3, HC11 and HC11 retrotransposition-positive clone cells. Arrows indicate E-cadherin-, N-cadherin- and Vimentin-antibody reactions. GAPDH refers to sample protein load. (C) Immunofluorescence of control HC11 and HC11/cl.19 cells after staining with E-cadherin and fibronectin antibodies as well as propidium iodide (PI). Scale bar, 20 µm. Data in (B and C) are representative of 3 experiments.