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. 2020 Apr 28;44(1):126–138. doi: 10.3892/or.2020.7596

Figure 3.

Figure 3.

RNA expression levels of EMT-associated genes in HC11 retrotransposition-positive cells. Total RNA isolated from either control HC11 or retrotransposition-positive massive clone cells subjected to Real-Time PCR analysis for examining the expression of EMT-transcriptional factors using designed primers (please see Materials and methods). Columns, corresponding to each transcription factor, represent the mean value of fold-mRNA expression from three independent experiments with ±SD indicated with bars in comparison to basal-control HC11 expression considered as 1-fold. C1-C4 and Mass. denote control HC11 and massive clone cells per respective analysis. GAPDH expression was used for cDNA template normalization. Presented P values were calculated using the paired sampler Student's t-test.