Figure 5.

Systemic Flagellin Treatment Enhances Uptake of FimH + Bacteria into Aged Peyer's Patches

(A–E) Aged mice (n = 3–4) were treated with flagellin (or PBS, control) and GFP-expressing E. coli K12 or Salmonella Typhimurium injected into ligated loops containing a Peyer's patch; 1.5 h later the abundance of these bacteria in the SED of Peyer's patches and MLN was quantified.

(A) IHC detection of GFP-expressing E. coli K-12 (arrows) in the SED. Nuclei detected using DAPI (blue). Broken line, apical FAE surface. Scale bar, 50 μm.

(B) Quantitation of the number of GFP-expressing E. coli K-12 in the SED of PBS- and flagellin-treated mice. Each point is from an individual section. Horizontal line, median. n = 36–45/group from 3 to 4 mice. Statistical difference determined by Mann-Whitney.

(C) IHC detection of GFP-expressing S. Typhimurium ΔaroA (arrows) in the SED. Nuclei detected using DAPI (blue). Broken line, apical FAE surface. Scale bar, 50 μm.

(D) Quantitation of the number of GFP-expressing S. Typhimurium ΔaroA in the SED of PBS- and flagellin-treated mice. Each point is from an individual section. Horizontal line, median. n = 67–171/group from 3 to 4 mice. Statistical difference determined by Mann-Whitney.

(E) Quantitation of S. Typhimurium ΔaroA colony-forming units (CFU)/g in MLN of PBS- and flagellin-treated mice. Points are MLN from individual mice. Horizontal line, median. n = 3–4/group. Statistical difference determined by t test.