Table 3.
Top 15 GO terms and pathways enriched for DEGs.
| Category | Term | Count | P | Genes |
|---|---|---|---|---|
| Rectome FI BP All | IL-18-mediated signaling pathway | 3 | 1.74 × 10−6 | IL18RAP, AKT1, IL18R1 |
| Rectome FI BP All | Apoptotic process | 11 | 8.80 × 10−6 | ARRB1, PHLDA1, MEF2A, PRKCE, PLSCR1, STK4, BCLAF1, MAP2K6, GADD45A, MAPK14, PKN2 |
| Rectome FI BP All | Protein phosphorylation | 10 | 1.06 × 10−5 | PRKAG2, RPS6KA5, AKT1, JAK1, PRKCE, IRAK3, STK4, CDK19, CSNK1A1, PKN2 |
| Rectome FI BP All | LPS-mediated signaling pathway | 4 | 1.25 × 10−5 | AKT1, PRKCE, TLR4, MAPK14 |
| Rectome FI BP All | Intracellular signal transduction | 9 | 1.62 × 10−5 | PRKAG2, RPS6KA5, AKT1, JAK1, WSB1, PRKCE, STK4, MAPK14, PKN2 |
| DAVID BP Up | Sulfur compound biosynthetic process | 6 | 2.17 × 10−5 | TPK1, MAT2A, EXT1, GCLM, HS3ST3B1, MTRR |
| PANTHER BP Up | Glycolysis | 4 | 2.31 × 10−5 | HK3, ENO1, PFKFB2, PKM |
| Rectome FI BP All | Positive regulation of peptidyl-serine phosphorylation | 5 | 2.59 × 10−5 | ARRB1, AKT1, CD44, STK4, ANGPT1 |
| DAVID BP All | Sulfur compound biosynthetic process | 7 | 2.76 × 10−5 | TPK1, MAT2A, CHST11, EXT1, GCLM, HS3ST3B1, MTRR |
| PANTHER BP Up | Phosphate-containing compound metabolic process | 22 | 2.98 × 10−5 | RAB20, MAPK14, ATP6V1C1, ATP11B, HK3, SGMS2, CSNK1A1, PRKCE, ATP9A, TPK1, PDGFC, MAPK6, GK, SGSH, IRAK3, GADD45A, GRPEL1, ARFGEF1, ATP1B3, MAP2K6, PPA2, PKM |
| Rectome FI BP All | Peptidyl-serine phosphorylation | 6 | 3.21 × 10−5 | AKT1, PRKCE, STK4, CSNK1A1, MAPK14, PKN2 |
| Rectome FI BP Up | LPS-mediated signaling pathway | 3 | 4.00 × 10−5 | PRKCE, MAPK14, TLR4 |
| Rectome FI BP All | IL-1-mediated signaling pathway | 3 | 4.55 × 10−5 | RPS6KA5, IRAK3, EGR1 |
| Rectome FI BP Up | Apoptotic process | 7 | 5.11 × 10−5 | PHLDA1, MAP2K6, MEF2A, GADD45A, PRKCE, MAPK14, PLSCR1 |
| Rectome FI BP Up | IL-18-mediated signaling pathway | 2 | 5.34 × 10−5 | IL18RAP, IL18R1 |
| Rectome FI MF All | Protein serine/threonine kinase activity | 9 | 1.26 × 10−5 | RPS6KA5, AKT1, PRKCE, IRAK3, STK4, MAP2K6, CSNK1A1, MAPK14, PKN2 |
| PANTHER MF All | Catalytic activity | 70 | 1.53 × 10−5 | CTSS, RAB20, MAPK14, DYRK2, ATP6V1C1, P2RY10, DTX4, POR, GYG1, TRIOBP, FKBP5, ATP11B, PRKAG2, HK3, SGMS2, PPP1R12B, PCMT1, HP, IMPA2, CSNK1A1, QSOX1, TLK1, CDK19, PRKCE, UPP1, CNOT6L, ATP9A, PNPLA6, CHST11, ARG1, NOV, MTRR, TPK1, CYSLTR1, PDGFC, CYB561, MAPK6, AKT1, GK, SGSH, CHI3L1, NUDT3, ENO1, PFKFB2, C3AR1, MAT2A, IRAK3, VNN1, GADD45A, RABGAP1L, GRPEL1, ARFGEF1, ATP1B3, SET, DICER1, SPDYE1, PARP8, MAP2K6, FUT4, PKN2, EXT1, GCLM, STK4, RPGRIP1, ERCC1, DCUN1D1, MSRB2, TOPORS, PPA2, PKM |
| PANTHER MF Up | Transferase activity | 20 | 2.09 × 10−5 | GYG1, HK3, SGMS2, PCMT1, CSNK1A1, TLK1, PRKCE, UPP1, TPK1, MAPK14, PDGFC, MAPK6, GK, MAT2A, IRAK3, GADD45A, MAP2K6, FUT4, EXT1, PKM |
| PANTHER MF All | Kinase activity | 19 | 3.00 × 10−5 | MAPK14, DYRK2, PRKAG2, HK3, CSNK1A1, TLK1, CDK19, PRKCE, PDGFC, MAPK6, AKT1, GK, IRAK3, GADD45A, SPDYE1, MAP2K6, PKN2, STK4, PKM |
| PANTHER MF All | Protein kinase activity | 15 | 3.19 × 10−5 | MAPK14, DYRK2, CSNK1A1, TLK1, CDK19, PRKCE, PDGFC, MAPK6, AKT1, IRAK3, GADD45A, SPDYE1, MAP2K6, PKN2, STK4 |
| PANTHER MF Up | Catalytic activity | 41 | 4.02 × 10−5 | RAB20, MAPK14, ATP6V1C1, POR, GYG1, FKBP5, ATP11B, HK3, SGMS2, PCMT1, HP, CSNK1A1, QSOX1, TLK1, PRKCE, UPP1, ATP9A, PNPLA6, ARG1, MTRR, TPK1, PDGFC, MAPK6, GK, SGSH, ENO1, PFKFB2, C3AR1, MAT2A, IRAK3, VNN1, GADD45A, GRPEL1, ARFGEF1, ATP1B3, MAP2K6, FUT4, EXT1, GCLM, PPA2, PKM |
| PANTHER MF All | Transferase activity | 29 | 1.09 × 10−4 | MAPK14, DYRK2, GYG1, PRKAG2, HK3, SGMS2, PCMT1, CSNK1A1, TLK1, CDK19, PRKCE, UPP1, CHST11, TPK1, PDGFC, MAPK6, AKT1, GK, MAT2A, IRAK3, GADD45A, SPDYE1, PARP8, MAP2K6, FUT4, PKN2, EXT1, STK4, PKM |
| Rectome FI MF Up | IL-1 receptor activity | 2 | 1.11 × 10−4 | IL1R2, IL18R1 |
| PANTHER MF Up | Kinase activity | 12 | 1.31 × 10−4 | MAPK14, HK3, CSNK1A1, TLK1, PRKCE, DGFC, APK6, GK, IRAK3, GADD45A, MAP2K6, PKM |
| Rectome FI MF All | ATP binding | 15 | 2.25 × 10−4 | PRKAG2, HK3, RPS6KA5, AKT1, JAK1, PRKCE, IRAK3, PKM, PFKFB2, STK4, MAP2K6, CDK19, CSNK1A1, MAPK14, PKN2 |
| Rectome FI MF Up | MAPK activity | 2 | 3.80 × 10−4 | MAP2K6, MAPK14 |
| PANTHER MF Up | Protein kinase activity | 9 | 3.93 × 10−4 | MAPK14, CSNK1A1, TLK1, PRKCE, PDGFC, MAPK6, IRAK3, GADD45A, MAP2K6 |
| Rectome FI MF All | Kinase activity | 4 | 4.13 × 10−4 | AKT1, PKM, CSNK1A1, PKN2 |
| Rectome FI MF All | IL-1 receptor activity | 2 | 5.01 × 10−4 | IL1R2, IL18R1 |
| DAVID MF Up | ATPase activity, coupled with transmembrane movement of ions, phosphorylative mechanism | 5 | 5.09 × 10−4 | ATP6V1C1, ATP1B3, ATP9A, ATP11B, ATP13A3 |
| Rectome FI CC All | Nucleus | 31 | 1.98 × 10−4 | ARRB1, ETS2, RPS6KA5, NFKBIZ, AKT1, PHLDA1, JAK1, KLF10, MEF2A, ARG1, PRKCE, IRAK3, CBFA2T3, PLSCR1, PKM, PFKFB2, DCUN1D1, UBR2, STK4, BCLAF1, GRPEL1, PDGFC, MAP2K6, EGR1, CDK19, GADD45A, MAPK14, COPS3, NFIC, PKN2, ZNF652 |
| Rectome FI CC All | Cytosol | 31 | 3.84 × 10−4 | PRKAG2, ARRB1, ETS2, HK3, GRB10, AKT1, PHLDA1, JAK1, MEF2A, WSB1, IL15, ARG1, PRKCE, GAB1, PLSCR1, PKM, PPP1R12B, CD44, PFKFB2, DCUN1D1, UBR2, STK4, MYL12A, PDGFC, MAP2K6, CDK19, CSNK1A1, MAPK14, COPS3, PKN2, LIMS1 |
| DAVID CC All | Golgi apparatus | 21 | 4.97 × 10−4 | SLC9A7, SGMS2, AGFG1, VPS41, IL15, CBFA2T3, ST6GALNAC2, SYNE1, CD44, ARRB1, ST8SIA4, CHST11, NAB1, PHTF1, FUT4, PDGFC, PRNP, EXT1, QSOX1, RAB20, HS3ST3B1 |
| Rectome FI CC Up | Nuclear speck | 5 | 6.15 × 10−4 | NFKBIZ, CSNK1A1, GADD45A, IL15, MAPK14 |
| DAVID CC All | Golgi apparatus part | 11 | 8.34 × 10−4 | SLC9A7, SGMS2, ARRB1, ST8SIA4, PHTF1, VPS41, PDGFC, CBFA2T3, EXT1, QSOX1, ST6GALNAC2 |
| DAVID CC Up | Golgi apparatus | 14 | 1.54 × 10−3 | AGFG1, SGMS2, IL15, SYNE1, CD44, NAB1, PHTF1, FUT4, PDGFC, EXT1, PRNP, QSOX1, RAB20, HS3ST3B1 |
| Rectome FI CC Up | Secretory granule lumen | 3 | 1.57 × 10−3 | HK3, MAPK14, PKM |
| Rectome FI CC Up | Ficolin-1-rich granule lumen | 3 | 1.97 × 10−3 | HK3, MAPK14, PKM |
| Rectome FI CC Up | Cell surface | 5 | 2.33 × 10−3 | PDGFC, IL15, ITGA7, TLR4, CD44 |
| DAVID CC All | Golgi membrane | 8 | 2.90 × 10−3 | SGMS2, ARRB1, ST8SIA4, PDGFC, CBFA2T3, EXT1, QSOX1, ST6GALNAC2 |
| DAVID CC Up | Endomembrane system | 12 | 5.66 × 10−3 | SYNE1, CYP1B1, SGMS2, AGFG1, LDLR, ATP11B, PDGFC, ASPH, EXT1, QSOX1, PNPLA6, PHLDA1 |
| DAVID CC All | Endomembrane system | 17 | 5.78 × 10−3 | CYP1B1, SGMS2, AGFG1, LDLR, ATP11B, TREX1, CBFA2T3, ST6GALNAC2, PNPLA6, SYNE1, ARRB1, ST8SIA4, PDGFC, ASPH, EXT1, QSOX1, PHLDA1 |
| DAVID CC Down | Golgi apparatus part | 6 | 7.84 × 10−3 | SLC9A7, ARRB1, ST8SIA4, VPS41, CBFA2T3, ST6GALNAC2 |
| DAVID CC All | Integral to organelle membrane | 6 | 8.37 × 10−3 | SGMS2, ST8SIA4, ASPH, EXT1, QSOX1, ST6GALNAC2 |
| DAVID CC All | Integral to Golgi membrane | 4 | 9.43 × 10−3 | SGMS2, ST8SIA4, QSOX1, ST6GALNAC2 |
| Rectome FI Pathway All | IL-6-mediated signaling events (N) | 6 | 8.42 × 10−8 | AKT1, JAK1, GAB1, IL6ST, MAP2K6, MAPK14 |
| Rectome FI Pathway Up | IL-12-mediated signaling events (N) | 4 | 8.61 × 10−6 | IL18RAP, MAP2K6, MAPK14, IL18R1 |
| Rectome FI Pathway Up | IL-1 family signaling (R) | 5 | 9.64 × 10−6 | IL18RAP, MAP2K6, IL1R2, IRAK3, IL18R1 |
| Rectome FI Pathway All | TNF-α signaling pathway (K) | 6 | 1.11 × 10−5 | RPS6KA5, AKT1, IL15, MAP2K6, MAPK14, IL18R1 |
| Rectome FI Pathway Up | TLR cascades (R) | 5 | 1.18 × 10−5 | MAP2K6, MEF2A, IRAK3, MAPK14, TLR4 |
| Rectome FI Pathway Up | Cytokine–cytokine receptor interaction (K) | 6 | 1.65 × 10−5 | IL18RAP, PDGFC, IL15, IL1R2, IL6ST, IL18R1 |
| Rectome FI Pathway All | Trk receptor signaling mediated by the MAPK pathway (N) | 4 | 1.69 × 10−5 | RPS6KA5, MAP2K6, EGR1, MAPK14 |
| Rectome FI Pathway Up | Myogenesis (R) | 3 | 1.93 × 10−5 | MAP2K6, MEF2A, MAPK14 |
| Rectome FI Pathway All | MAPK signaling pathway (K) | 8 | 1.93 × 10−5 | ARRB1, RPS6KA5, AKT1, IL1R2, STK4, MAP2K6, GADD45A, MAPK14 |
| Rectome FI Pathway Down | IL-6-mediated signaling events (N) | 3 | 2.06 × 10−5 | GAB1, AKT1, JAK1 |
| Rectome FI Pathway All | CXCR3-mediated signaling events (N) | 4 | 2.13 × 10−5 | ARRB1, AKT1, MAP2K6, MAPK14 |
| Rectome FI Pathway Up | p38 MAPK signaling pathway (N) | 3 | 2.74 × 10−5 | MAP2K6, GADD45A, MAPK14 |
| Rectome FI Pathway All | TLR cascades (R) | 6 | 4.80 × 10−5 | RPS6KA5, MEF2A, IRAK3, TLR4, MAP2K6, MAPK14 |
| Rectome FI Pathway Up | Trk receptor signaling mediated by the MAPK pathway (N) | 3 | 4.96 × 10−5 | MAP2K6, EGR1, MAPK14 |
| Rectome FI Pathway Up | IL-1-mediated signaling events (N) | 3 | 5.42 × 10−5 | MAP2K6, IL1R2, IRAK3 |
All: all candidate DEGs; BP: biological process; CC: cellular component; Down: down-regulated candidate DEGs; MF: molecular function; Up: up-regulated candidate DEGs.