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. 2020 May 27;39:94. doi: 10.1186/s13046-020-01592-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Identification of circ_0008532 in bladder cancer. a-b The Venn and schematic diagrams show that five exons derived from MTGR1 constitute circ_0008532. The existence of circ_0008532 was demonstrated by PCR and its back-splicing junction was verified by Sanger sequencing. c The expression level of Circ_0008532 in 10 pairs of bladder cancer and adjacent non-tumor tissue. d The expression level of Circ_0008532 in normal bladder urothelial cells (NBUCs), human uroepithelial cells (SV-HUC-1) and bladder cancer cell lines (EJ, T24T, UM-UC-3, TCCSUP, T24, 5637, SCABER, SW780 and J82). e PCR assay with divergent or convergent primers indicating circ_0008532 is present in the EJ and T24 cell lines. GAPDH was used as a negative control. f Real-time qPCR analysis of the expression of circ_0008532 after RNase R treatment in EJ or T24 cells. g RNA-FISH indicates the location of circ_0008532. Nuclei were stained blue with DAPI. Circ_0008532 is stained red with cy3. Bar graphs show the statistical analysis of three independent experiments (* P < 0.05)