Figure 2.

CDYL2 Over-Expression in the Non-invasive Breast Cancer Line MCF7 Induces Transcriptional Changes Associated with Malignant Progression

(A) Western blot analysis of CDYL2 and beta-actin expression in MCF7-CDYL2 and MCF7-Vector cells.

(B) Volcano plot showing genes up- or down-regulated at least 2.5-fold at an adjusted p value less than 0.05 (t test).

(C) Selected molecular signatures over-represented in either the up-or down-regulated gene sets from (B).

(D) Heatmap showing expression of genes from molecular signatures in (C) in the triplicate RNA-seqs.

(E) qRT-PCR validation of selected differentially expressed genes from (C). Mean of three independent experiments ±S.D. All differential expressions were significant at p < 0.05 (t test).