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. 2020 May 12;23(6):101152. doi: 10.1016/j.isci.2020.101152

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Ecc Aversion Does Not Require the IMD/NF-κB Signaling Pathway

(A and B) Heat-killed Ecc is no longer aversive to adult females. (A) (Top) Kinetics of the AI for sucrose when flies were given the choice between a heat-killed Ecc + sucrose versus sucrose-only solution. (Bottom) CAI area for each specified solution (arrows) and its distribution over time. (B) (Left): Histograms built with the CAI values from (A). ∗p < 0.05. Wilcoxon matched-pairs signed rank test, two-tailed p value. Error bars correspond to standard deviation. The reference index for boiled Ecc calculated with the CAI values from (A).

(C) PGRP-LB, Diptericin-Cherry flies fed for 24 h with either fresh (left) or boiled Ecc (right). Scale bar, 0.5 mm.

(D and E) Relish mutant flies behaved like controls. (D) (Top) Kinetics of the AI for sucrose when rel^E20/Df(3R)ED5301 flies and control rel^E20/+ and Df(3R)ED5301/+ flies were given the choice between a Ecc + sucrose solution versus sucrose only. (Bottom) CAI area for each of the specified solution (arrows) and its distribution over time. (E) Histograms built with the CAI values from (D). ∗p < 0.05 and ∗∗p < 0.01. Wilcoxon matched-pairs signed rank test, two-tailed p value. Error bars correspond to standard deviation. The preference index for Ecc is calculated with the CAI values from (D).

(F and G) Flies mutant for the amidase PGRP-LB displayed a normal two-step behavior regarding Ecc. (F) (Right) Kinetics of the AI for sucrose when PGRP-LB^Δ flies were given the choice between an Ecc + sucrose solution versus sucrose only. (Left) CAI area for each specified solution (arrows) and its distribution over time. (G) Histograms built with the CAI values from (F). ∗∗p < 0.01. Wilcoxon matched-pairs signed rank test, two-tailed p value. Error bars correspond to standard deviation. The preference index for Ecc is calculated with the CAI values from (F). For (A and F) left graphs and (D) top graphs, the black lines and the gray lines correspond, respectively, to the mean and the standard deviation, and for (A and D) right graphs and (C) bottom graphs, solely the mean value of the CAI obtained with multiple replicates is shown in black. n indicates the number of experimental replicates. a.u.: arbitrary unit. Data are represented as mean ± SD.