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. Author manuscript; available in PMC: 2021 Jan 3.
Published in final edited form as: Circ Res. 2019 Oct 28;126(1):41–56. doi: 10.1161/CIRCRESAHA.119.315932

Figure 7. Poldip2 deficiency induces inhibition of UPS by an OGT-dependent mechanism.

Figure 7.

A) Proteasome activity in HASMCs transfected in control and Poldip2-deficient cells transfected with control siRNA or against OGT was measured using a fluorogenic substrate as described in the methods section. B) Immunoprecipitation to evaluate the accumulation of poly-ubiquitinated proteins, and the Ub-GFP reporter in Poldip2-deficient cells treated with siRNA control or against OGT. Scatter plots and mean from 4–5 independent experiments are shown. P value indicated was calculated by two-way ANOVA, * < 0.05, ***<0.001.