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. 2020 May 19;11:425. doi: 10.3389/fphys.2020.00425

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Copyright © 2020 Yang, Wang, Zhang, Cai, Jiang, Zha and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A PK2 antagonist and an AKT inhibitor blocked the effects of Met on HG-treated cardiomyocytes. (A) Images of PK2, PKR1, and PKR2 protein levels. (B) Analysis of PK2. (C) Analysis of PKR1. (D) Analysis of PKR2. (E) Images of p-AKT and AKT protein levels. (F) Analysis of p-AKT. (G) Analysis of AKT. (H) Analysis of the p-AKT/AKT ratio. (I) Images of p-GSK3β and GSK3β protein levels. (J) Analysis of p-GSK3β. (K) Analysis of GSK3β. (L) Analysis of the p-GSK3β/GSK3β ratio. (M) Images of Bcl-2 and Bax protein levels. (N) Analysis of Bax. (O) Analysis of Bcl-2. (P) Analysis of the Bax/Bcl-2 ratio. The data are mean ± SEM; * P value less than 0.05 versus the NG group; ^# P value less than 0.05 versus the HG group; n = 3–4 per group.