Skip to main content
. 2019 Aug 27;2019:10.17912/micropub.biology.000146. doi: 10.17912/micropub.biology.000146

Figure 1.

Figure 1

Overview of plasmids for mosSCI at ttTi5605-compatible sites using G418 (NeoR) (A) or unc-119(+) (B) selection cassettes flanked by loxP sites. Both plasmids are modified from pCFJ151 (ttTi5605-MCS) (Frokjaer-Jensen et al. 2008). Results of test injections (three different transgene constructs for each plasmid) are shown below the diagrams. The mosSCI “direct injection protocol” was followed, using Phsp::peel-1 and three mCherry(+) co-injection markers to screen against extrachromosomal arrays as described (Frokjaer-Jensen et al. 2012)(www.wormbuilder.org). Transgenes in pALS297/NeoRor pALS186/unc-119(+) were used for mosSCI injections into strains ALS344 or EG6699, respectively (Table 1).