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. 2019 Jun 7;116(3):658–670. doi: 10.1093/cvr/cvz148

Figure 2.

Figure 2

MYH6:mCherry+ cells express cardiac markers and display well-organized sarcomeric structure. (A) MYH6:mCherry+ cells co-express cardiac markers MYH6, MYL7, MYL2, TNNT2, GATA4, and ACTN2 at differentiation Day 15. In the merged images, MYH6:mCherry are shown in red and individual cardiac makers are coloured by green. Scale bar: 100 µm, except for ACTN2: 10 µm. (B) Flow cytometry analysis of MYH6:mCherry+ CMs with co-expressed cardiac markers. (C) Transmission electron micrograph demonstrating the sarcomeric structures in MYH6:mCherry+ CMs. Red arrows indicate Z-lines. (D) Expression of cardiac genes in MYH6:mCherry sorted cells. Results represent data from three independent experiments. A two-tailed Student’s t-test was used to calculate statistical significance. Error bars show SD. Statistical significance is indicated: ∗∗∗P < 0.001.