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. 2019 Jun 7;116(3):658–670. doi: 10.1093/cvr/cvz148

Figure 4.

Figure 4

ESC-derived MYH6:mCherry+ cells display cardiac gene expression profiles. (A) A representative FACS plot of MYH6:mCherry+ and MYH6:mCherry− cell populations. Cells were harvested at differentiation Day 20. (B) Hierarchical clustering analysis of altered gene expression based on RNA sequencing from MYH6:mCherry+ and MYH6:mCherry− populations. (C) PCA analysis of the transcriptome of MYH6:mCherry+ and MYH6:mCherry− cells and previously published hESC-derived cardiomyocytes (GSE93841). GSE93841-MM: hESC-derived CMs cultured in maturation medium; GSE93841-control: hESC-derived cells cultured in control medium; (D) GSEA analysis indicates that genesets of two independent MYH6:mCherry+ samples (MHC1 and MHC2) are both significantly enriched in adult human right/left atrial and ventricular cells compared to hESC-CMs (at 8 weeks). A published microarray dataset (GSE64189) and the genesets that are >10-fold up-regulated from MYH6:mCherry+ populations were used for comparison. Enriched genesets are selected based on statistical significance (FDR q value <0.25 and/or NOM P value <0.05). MHC 1 and MHC2: two independent MYH6:mCherry+ samples. RA/LA: human adult right/left atrium. RV/LV: human adult right/left ventricular cells. hESC-CM (8 weeks): hESC-derived cardiomyocytes harvested at differentiation Week 8. (E) GO biological process analyses of the genes that are significantly enriched in human adult right/left atrial samples. Sorted cells are from two independent experiments.