Figure 3.

Bcl-2 and Beclin 1 protein expression levels in untreated cells. Western blot detection of proteins from whole cell lysates using 30 µg protein loading of U-937, AML MOLM-13 and OCI-AML2 cells and CML K562. (A) Representative data image of a western blot. β-actin was used as a housekeeping protein. Basal protein expression of (B) anti-apoptotic Bcl-2 and (C) autophagy Beclin 1. Data are expressed as the mean ± SE; n=4. One-way ANOVA; Tukey's post-hoc test was used for grouping and pairwise comparison. A two-sample t-test was used to compare the Bcl-2 isoforms in the MOLM-13 cell line.