Skip to main content
. 2020 Apr 13;57(1):139–150. doi: 10.3892/ijo.2020.5043

Figure 1.

Figure 1

Expression of GSG2 in PCa tissues GSG2 and construction of a GSG2-knockdown cell model. (A) Representative immunohistochemical staining results: Low expression of GSG2 in adjacent normal tissues and high expression of GSG2 in PCa tissues (magnification, x100). (B) Transfection efficiency was examined by fluorescence imaging in PC-3 and DU 145 cells (magnification, x200). (C) GSG2 mRNA expression was determined by reverse transcrip tion-quantitative PCR in PC-3 and DU 145 cells. (D) GSG2 protein expression level was detected by western blotting in PC-3 and DU 145 cells. ***P<0.001. GSG2, germ cell-specific gene 2; PCa, prostate cancer; shGSG2, cells transfected with GSG2-targeting short hairpin RNA; shCtrl, cells transfected with the control short hairpin RNA.