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. 2020 Apr 8;57(1):197–212. doi: 10.3892/ijo.2020.5041

Figure 6.

Figure 6

MAPK signaling pathway contributed to MMP2 overexpression in clear cell renal cell carcinoma. (A) MMP2 mRNA expression was determined by reverse transcription quantitative PCR in Caki-1 cells treated with the MAPK signaling inhibitors U0126, SB203580 or SP600125 for 24 h. U6 was used as an internal control. (B-D) Western blotting of (B) P-ERK, ERK, p-ERK/ERK and MMP2, (C) p-p38, p38, p-p38/p38 and MMP2 and (D) p-JNK, JNK, p-JNK/JNK and MMP2 in Caki-1 cells after treatment with U0126, SB203580 or SP600125, respectively. GAPDH was used as an internal control. Each analysis was performed at least three times and each sample assessed in triplicate. Data were expressed as the means ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001 vs. DMSO. ns, not significant; MMP2, matrix metalloproteinase 2; ns, not significant; p, phosphorylated.