Skip to main content
. 2020 Apr 27;57(1):264–276. doi: 10.3892/ijo.2020.5055

Figure 3.

Figure 3

miR-508-3p suppresses CCNA2/pRb/CDK4/6 signaling and MMP7 levels, and CCNA2 is involved in miR-508-3p-induced inhibition of the cell cycle. (A) Western blot assay results demonstrated that miR-508-3p attenuated pRb, CDK6 and MMP2 protein levels. (B) Detection of MMP7 levels in the supernatant of ovarian cancer cells transfected with miR-508-3p or miR-ctrl by ELISA. (C-E) SKOV3 cells were transfected with siRNA-ctrl or siRNA-CCNA2, and the (C) protein level, (D) cell proliferation and (E) percentage of cells in each phase of the cell cycle were assessed by western blot, MTT and flow cytometry assays, respectively. (F-H) SKOV3 cells were co-transfected with CCNA2 vector or EV with miR-508-3p or miR-ctrl, and the (F) protein level, (G) cell proliferation and (E) percentage of cells in the G1 phase were assessed by western blot, MTT and flow cytometry assays, respectively. Kruskal-Wallis test was used to analyze the data. *P<0.05, **P<0.01, ***P<0.001. miR, microRNA; ctrl, control; CCNA2, cyclin A2; MMP7, matrix metalloproteinase 7; siRNA, small interfering RNA; EV, empty vector.