Figure 1. USP7 interacts with EZH2. (A) HA-EZH2 was expressed in HEK293T cells with Flag-USP7 or Flag-USP7 C223S. Cell lysates were immunoprecipitated with anti-Flag antibody and immunoblotted with anti-Flag or anti-HA antibody. (B) Endogenous interaction of USP7 and EZH2. DU145 cell lysates were immunoprecipitated with normal IgG or anti-EZH2 antibody and immunoblotted with anti-USP7 or anti-EZH2 antibody. (C) Immunofluorescence staining of USP7 and EZH2 in DU145 or PC3 cells. USP7 was stained green and EZH2 was stained red. The blue signal represents nuclear DNA stained by DAPI. The bar indicates 10 μm. (D) Schematic representation of the USP7 deletion mutants. TRAF-like, TNF receptor-associated factor (TRAF)-like N-terminal domain; CD, catalytic domain; Ubl, Ub-like domain. (E) HA-EZH2 was expressed in HEK293T cells in combination with each of the Flag-tagged USP7 deletion mutants. Cell lysates were immunoprecipitated with anti-Flag antibody and immunoblotted to detect HA-EZH2 and Flag-tagged USP7 deletion mutants.
