Cola5IV in podocytes. a Cola5IV mRNA in NG (5.5 mM glucose), HG (40 mM glucose) and HG + ALI (40 mM glucose + 20 mM ALI), n = 3. b Cola5IV protein in NG (5.5 mM glucose), HG (40 mM glucose) and HG + ALI (40 mM glucose + 20 mM ALI), n = 6. c In NG, ALI did not increase Cola5IV protein, nor was Cola5IV protein changed by isotonic mannitol (5.5 mM glucose + 34.5 mM mannitol, n = 3 in each group). ** p < 0.05 vs. NG, * p < 0.05 vs. HG.