24-hour MMP2 in podocytes. a MMP2 mRNA in NG (5.5 mM glucose), HG (40 mM glucose) and HG + ALI (40 mM glucose + 20 mM ALI), n = 3. b Activated MMP2 protein in NG (5.5 mM glucose), HG (40 mM glucose) and HG + ALI (40 mM glucose + 20 mM ALI), n = 3. c In NG, ALI did not reduce MMP2 protein, nor was MMP2 protein changed by isotonic mannitol (5.5 mM glucose + 34.5 mM mannitol, n = 3 in each group).