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. 2020 May 7;11:832. doi: 10.3389/fmicb.2020.00832

FIGURE 2.

FIGURE 2

SAB-159 neutralizes MAb escape mutants. Pseudovirions with wildtype GnGc, single MAb escape mutations, or a double escape mutation were used in PsVNA to determine the effect mutations have on the capacity of antibody to neutralize virion entry into cells. (A) Anti-HTNV MAb 3D5, MAb HCO2, and a 1:1 MAb cocktail of those antibodies were evaluated in the PsVNA. The IC50 were plotted. (B) The anti-HTNV purified human IgG (SAB-159) prediluted at 1:30 and control polyclonal antibodies were evaluated by PsVNA. The PsVNA50 titers were plotted. The positive control was anti-HTNV rabbit (rab) sera; the negative control was normal rabbit sera; and the negative control was purified IgG antibody from a naïve TcB (Negative IgG). *Indicates that the mutation(s) in the GnGc resulted in a >10-fold reduction in PsVNA50 titer (escape). Limit of quantitation is 20 (gray shaded area).