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. 2020 May 27;16(5):e1008539. doi: 10.1371/journal.ppat.1008539

Fig 4. The NAD salvage pathway controls metabolism and survival of immature S. mansoni.

Fig 4

(A) Intracellular NAD levels in S. mansoni schistosomula cultured for 48h with vehicle or 250nM FK866 ± 2mM NMN or Trp. n = 6 wells/group with 2 schistosomula/well. (B) Representative light microscopy images of schistosomula 7 days post-treatment with vehicle or 250nM FK866 ± 2mM NMN or Trp. (C) Parasite mobility by S. mansoni schistosomula cultured for 10 days with vehicle or 250nM FK866 ± 2mM NMN or Trp. n = 3 wells/group with 45–60 schistosomula/well. (D) Survival of schistosomula cultured for 10 days with vehicle or 250nM FK866 ± 2mM NMN or Trp. Data are shown as the percentage of parasites alive at each timepoint. n = 45–60 parasites/group. Data are representative of 2 independent experiments. Data shown as the mean ± SD with individual samples in squares (A, C). Statistical tests were performed using one-way ANOVA multiple comparison tests or Log-rank (Mantel-Cox) test for survival.