Figure 2.

Method repeatability. Two different standards, that is, pooled human serum (N = 48) and commercially available pooled human plasma (N = 40), were randomly distributed over eight 96-well plates and underwent the full sample preparation and analysis workflow. Filled bars show the mean and error bars show the SD of relative intensities for the three most abundant glycopeptides after total area normalization per each of the 11 different peptides (clusters). Monosaccharide symbols: blue square = N-acetylglucosamine; green circle = mannose; yellow circle = galactose; pink diamond = sialic acid; red triangle = fucose; yellow square = N-acetylgalactosamine (see also Figure 1).