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. 2020 May 7;5(9):e133497. doi: 10.1172/jci.insight.133497

Figure 7. SERCA2B, RyR2, and AC9 expression is altered by T cell exosomes in 3D cultures of HSG acini.

Figure 7

(A) miR-142-3p expression in 3D HSG acini that were treated with complete epithelial cell medium (control), pure exosomes isolated from activated T cells, or pure exosomes isolated from activated T cells and miR-142-3p hairpin inhibitor. (B) Western blots of SERCA2B, RyR2, and AC9 in 3D HSG acini that were treated with complete epithelial cell medium (control) and pure exosomes isolated from activated T cells. (C) Graph shows relative protein levels of SERCA2B, RyR2, and AC9 in 3D HSG acini obtained by densitometric analysis. (D–F) Relative mRNA levels of SERCA2B, RyR2, and AC9 in 3D HSG acini that were treated with complete epithelial cell medium (control), pure exosomes isolated from activated T cells, or pure exosomes isolated from activated T cells and miR-142-3p hairpin inhibitor. (n = 5, median, maximum, and minimum shown; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, determined by Mann-Whitney nonparametric test.) The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range.