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. 2020 May 7;5(9):e128820. doi: 10.1172/jci.insight.128820

Figure 2. GCN2-KO mice exhibit glucose intolerance and reduced pancreatic β cell mass when fed a high-fat diet (HFD).

Figure 2

(A) Immunoblot analysis of GCN2 and β-actin in tissues of 12-week-old WT mice (upper) and the relative GCN2/β-actin ratio (lower). Data are the mean ± SEM (n = 4). Quantative data for 4 independent experiments are shown. (B) Immunoblot analysis of GCN2 in tissues from 12-week-old GCN2+/+, GCN2+/–, and GCN2–/– mice. (C–E) Blood glucose (C) and serum insulin concentrations (D) during an OGTT and immunostaining of insulin (red) and glucagon (green) in pancreatic sections (E, right) from 24-week-old normal chow diet–fed (NCD-fed) GCN2+/+ and GCN2–/– mice. Quantified pancreatic β cell mass (E, left). (F) Immunoblot analysis of phosphorylated and total GCN2 in the islets of 12- or 24-week-old WT mice fed a NCD or HFD. (G–I) Blood glucose (G), serum insulin levels (H) during an OGTT, and immunostaining of insulin (red) and glucagon (green) in pancreatic sections (I, right) from 24-week-old HFD-fed GCN2+/+ and GCN2–/– mice. Quantified pancreatic β cell mass (I, left). (J–L) Blood glucose (J), serum insulin levels (K) during an OGTT and immunostaining of insulin (red) and glucagon (green) in pancreatic sections (L, right) from 24-week-old HFD-fed βGCN2+/+ and βGCN2–/– mice. Quantified pancreatic β cell mass (L, left). Scale bars: 100 μm (E, I, and L ). Quantitative data in C–E and G–L are the mean ± SEM (n = 6–11). *P < 0.05, **P < 0.01 (2-tailed Student’s t test).