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. 2020 May 7;5(9):e135031. doi: 10.1172/jci.insight.135031

Figure 2. Circulating CD8+ T cells are more activated during flare when compared with remission and healthy donors.

Figure 2

(A) Identification of CD8+ T cells (previously gated as singlets, live, CD45+, CD3+) and subsequent gating of nonnaive CD8+ T cells lacking coexpression of CD45RA and CCR7. (B–D) Flow cytometric analyses of healthy donor (n = 10), iMCD-TAFRO flare (n = 9), and iMCD-TAFRO remission (n = 9) nonnaive CD8+ T cells with representative plots gating CD38+HLA-DR+ (B), PD-1+TIGIT+ (C), Ki67+ (D), and perforin+granzymeB+ CD8+ T cells (E) in flare and remission and comparison of frequencies across remission versus flare and healthy donor (HD) versus flare. Data are mean ± SEM. P values are based on paired 2-tailed t tests between remission and flare samples and unpaired 2-tailed t tests between healthy donor and flare with a Bonferroni’s correction for multiple comparisons. *P < 0.05; **P < 0.01.