Figure 4. Monocyte and NK cell subset composition is altered during flare when compared with healthy donors.

(A) Identification of CD56-expressing and CD16-expressing NK cell subsets, previously gated as singlets, live, CD45⁺, within the lymphocyte gate (SSC-A-low), CD3^–, and CD19^– during iMCD-TAFRO flare and remission. (B) Relative frequency of CD56^bright NK cells among all NK cells from healthy donors (n = 10), iMCD-TAFRO flare (n = 10), and iMCD-TAFRO remission (n = 10). (C) Identification of monocytes (previously gated as singlets, live, CD45⁺, within the monocyte gate [SSC-A-intermediate and CD4-intermediate] CD3^–, CD19^–, CD56^–) and subsequent gating of classical (CD14⁺CD16^–) and nonclassical (CD14^–CD16⁺) monocytes during iMCD-TAFRO flare and remission. (D) Ratio of classical to nonclassical monocytes from healthy donors (n = 10), iMCD-TAFRO flare (n = 10), and iMCD-TAFRO remission (n = 10). (E and F) The absolute number of classical (E) and nonclassical (F) monocytes per μL of whole blood across paired remission and flare samples. Data are mean ± SEM. P values are based on paired 2-tailed t tests between remission and flare samples, with a Bonferroni’s correction for multiple comparisons. **P < 0.01.