(A) Body weight (BW) change (vs. initial body weight) at time of sacrifice, expressed in grams; (B) muscle weights normalized to initial body weight (IBW); and (C) whole body grip strength assessment (expressed in grams) in CD2F1 male mice (8 weeks old) either injected with C26 tumor cells s.c. (1,000,000 cells/mouse in sterile PBS, C26) or intrasplenically (250,000 cells/mouse in sterile PBS, mC26). Control (Con for C26) and Sham (controls for mC26) were injected with an equal volume of vehicle (n = 4–6). Data are expressed as mean ± SD. One-way ANOVA was performed to determine differences between Con, C26, Sham, and mC26. #P < 0.05, ###P < 0.001, ####P < 0.0001 versus Con; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus Sham; $$P < 0.01, $$$P < 0.001 versus C26.