Figure 3. mC26 disrupts skeletal muscle anabolism.

Representative Western blotting and quantification (expressed as fold change versus Sham) for phospho-STAT3, STAT3, phospho-ERK1/2, ERK1/2, phospho-p38, p38, phospho-AKT, AKT, phospho-mTOR, mTOR, phospho-4EBP1, and 4EBP1 (blot 1) and for phospho-p70S6K and p70S6K (blot 2) in the muscle of CD2F1 male mice (12 weeks old) intrasplenically injected with C26 tumor cells (250,000 cells/mouse in sterile PBS, mC26) or an equal volume of vehicle (Sham) (n = 5). Tubulin was used as loading control in both blots. Quantification of phospho/total protein ratios are reported as mean ± SD. Two-tailed t tests were used to determine differences between Sham and mC26. *P < 0.05, **P < 0.01, ****P < 0.0001 versus Sham.