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. 2020 May 7;5(9):e136687. doi: 10.1172/jci.insight.136687

Figure 4. Increased protein catabolism in mC26 mice.

Figure 4

(A) Representative Western blotting and quantification (expressed as fold change versus Sham) for total ubiquitin and tubulin in CD2F1 male mice (12 weeks old) intrasplenically injected with C26 tumor cells (250,000 cells/mouse in sterile PBS, mC26) or an equal volume of vehicle (Sham) (n = 5). Tubulin was used as loading control. (B) Gene expression levels for Atrogin-1, MuRF-1, and Fbxo31 ubiquitin ligases measured by quantitative real-time PCR. Gene expression was normalized to TBP levels. Data are expressed as mean ± SD. Two-tailed t tests were used to determine differences between Sham and mC26. *P < 0.05, **P < 0.01, ***P < 0.001 versus Sham.